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PMCID: PMC6472203

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The microspheres combine all the advantages of affinity protein purification (low costs, simplicity, high specificity, and capacity) and magnetic properties to perform efficient manual or automatic quick high-throughput purification

Model establishment followed the reported protocol ( 2.3 Open field test (OFT) The OFT was conducted to assess anxiety-like behavior, exploratory activity, and locomotor function in mice

Outcomes include less insulin need, lower resistance, reduced fat buildup, and higher energy metabolism

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