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After three washes with 200 l prewarmed PBS, 100 l of 300 M prewarmed Hanks balanced salt solution (Gibco) was added, and luminescence was immediately measured at 37 C using a Synergy H1 reader
We also tested the sodA and spxB mutants that have previously shown a lower phenotype than wt [6, 32], although these strains displayed a higher H 2 O 2 resistance than the mentioned mutants, but significantly lower compared with the wt strain (Fig 3B, Fig S2)
However, deficiencies can occur in individuals who have difficulty absorbing nutrients or those who follow vegetarian or vegan diets without supplementation
Aim for seven to nine hours per night to support melatonin production and control cortisol levels, which can otherwise contribute to inflammation and break down collagen
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