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Efficacy plateaus above 2ml per injection because hepatic methylation pathways become saturated, and excess lipotropic compounds are simply excreted unchanged

Transfection procedures were performed using Lipofectamine 3000 (Invitrogen, USA), as per manufacturers instructions

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IGF-1 LR3 For In Vitro Research Use Only Not for Human or Veterinary Application For In Vitro Research Use Only Not for Human or Veterinary Application IGF-1 LR3 (Long Arg3 Insulin-like Growth Factor-1) is a synthetic 83-amino acid analog of native insulin-like growth factor-1 (IGF-1), incorporating a 13-amino acid N-terminal extension and a substitution of arginine for glutamic acid at position 3 of the mature IGF-1 sequence