An alternative interpretation for the first phase could be a rapid noncovalent interaction between PfGrx and GSSG in the vicinity of roGFP2, however, this seems less likely considering the smaller values for the structurally similar PfGrx mutants or, in particular, the rxYFP fusion constructs with ScGrx1 (Table 1)
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Make fresh solutions when possible
Sirolimus: its discovery, biological properties, and mechanism of action
~5% of APAP is metabolized via oxidation by the hepatic cytochrome P450 (mainly CYP2E1) into a toxic, highly reactive intermediate metabolite N-acetyl-p-benzoquinoneimine (NAPQI) *
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