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To assess cell viability, 10 l CellTiter-Fluor (Promega) was added to black plates and incubated for 30 min in the dark before fluorescence was measured at 7-mm depth (gain 100)

Established research on intranasal peptide delivery exists for oxytocin, insulin, and several neuropeptides

BB10 peptide is generally known as a dual-peptide research blend that combines two commonly studied peptides into one formulation

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J Am Coll Cardiol 60:10311042

Hydroxytyrosl is also reported to induce Nrf2 in PI3K dependent manner (Martn et al., 2010)