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The abnormal cold tolerance of the slcr-46.1 mutant was suppressed by interference with copper homeostasis and cuproptosis-signaling genes

Cuenca-Zamora, E

No enzymatic treatment was performed prior to the protein assay

This includes following specific directions on timing and frequency

While many lipid mediators result from these enzymatic processes, others such as phytoprostanes and aldehydes are created adventitiously when they encounter reactive oxygen species (ROS), and together, they are a redox regulatory network that couples ROS generation with subsequent stress signalling pathways in plants