The frequency depends on the indication and is set by a doctor
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It is responsible for producing melatonin, a hormone secreted by the pineal gland
But if you take supplements, you might ingest more than your body needs
That introduces measurement error
After incubation with the appropriate antibodies for 30 min, erythrocytes were lysed with lysis buffer (BD FACS Lysing Solution, BD Biosciences, San Jose, USA) for 10 min on a shaker, followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and washing with HBS
