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To investigate whether ROS was host or pathogen generated, a separate experiment was conducted in which cells were infected with 1 MOI zoospores (T#5), and either: 1) supplemented with 2.5 mM cysteine on day 3 for 8 hr, 2) incubated at 27C for 8 hr, 3) supplemented with 1.5 mM cysteine and incubated at 30C for 6 hr

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In Alzheimers, Parkinsons and other neurodegenerative diseases a local NAD+ deficit is observed in the affected neurons