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When stored properly, Trimix can last 35 to 45 days refrigerated after opening, while frozen Trimix can remain effective for up to a year
doi:10.1093/oxfordjournals.humrep.a019708 Lenzi A, Lombardo F, Gandini L, Culasso F, Dondero F
Lim C-F, Docter R, Visser TJ, Krenning EP, Bernard B, et al
Medical Journal of Australia, 170 (5), 218-218
In the brain, this imbalance can have particularly damaging effects due to its high metabolic demand, abundant lipid content, and relatively low antioxidant defences compared to other organs