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Stimulation of de novo glutathione synthesis by NFT concentrations in this range was observed in HepG2 cells, PHHs, or human neuronal cells, but not to the same extent in other cell types such as human iPSC-derived astrocytes

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After purification, islets were cultured overnight at 37 C in a humidified atmosphere of 95% air and 5% CO 2 in RPMI 1640 medium supplemented with 10% FBS, 100 units/ml penicillin and 0.1 mg/ml streptomycin