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Intracellular Ca 2+ mobilization assay FlpInCHO-hGLP-1R and FlpInCHO-pGLP-1R cells were seeded at a density of 3 10 4 cells/well into 96-well culture plates and incubated overnight at 37 C in 5% CO 2 and receptor-mediated intracellular Ca 2+ mobilization determined as described previously 49