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After three washes with free-serum media, cells were collected, counted, and analyzed for their fluorescence intensity using a fluorescence microplate reader (Thermo Fisher Scientific, Waltham, MA, USA) with its excitation and emission wavelengths at 488 nm and 525 nm [29, 30]

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As of December 29, the regulator had nine generic semaglutide submissions, including filings from Sandoz Canada, Apotex, Teva Canada, Taro Pharmaceuticals and Aspen Pharmacare Canada

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