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In vivo biological tests utilized C57BL/6 mice subjected to a subacute PD model induced by daily intravenous administration of 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) (40 mg/kg) for seven days

The choice always comes down to the goal of the study

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Male mice underwent rapid loss of survival at extended time points, while female mice were largely unaffected

One wonders whether precision cut liver slices have provided unique toxicological insights, or whether cross-species hepatocyte toxicity studies would have provided equally informative data had they been undertaken