The samples were fixed with 4% PFA at room temperature for 30 min, permeabilized with 0.1% Triton-100 at room temperature for 15 min, and blocked with 5% BSA solution at 37 C for 1 h
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formaldehyde resins
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However, using them in excess or combining them with incompatible products may disrupt the skin barrier, potentially leading to breakouts