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Insoluble immune complexes were rinsed once with Co-IP buffer then eluted by 0.1 mM citrate (pH3.0) twice for 5 min, resolved by sodium dodecyl sulfatepolyacrylamide gel, and transferred to nitrocellulose

Acknowledgements We express our gratitude to the Vice-Chancellor for Research, Isfahan University of Medical Sciences for financial support through the scientific code 398979 and grant number 1989096