The documented standard is 2mL of bacteriostatic water, which reconstitutes the 80mg vial to a 40mg/mL working concentration
In this situation, it can be challenging to demonstrate B12 deficiency even when there are typical symptoms (including neuropathy-like paraesthesia and numbness), as serum B12 concentrations may be just within, or sometimes above, the normal range due to the oral supplementation
For the oral spray, the recommended protocol involves one spray twice daily
Typical daily dose: 2,330 mcg (2.33 mg) total blend once daily for 4 weeks, then 24 weeks off
A positive ray spectrograph
This is why mastering this simple calculation isn't just 'good practice'it's a critical, non-negotiable element of rigorous scientific inquiry