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For sample preparation, the labeled stock solution was further diluted to obtain working solutions with concentrations suitable for spiking purposes in the range of 0.01 and 0.5 g/mL

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aureus biofilms skewed neutrophil to neutrophil NETs formation via the combined activity of the leukocyte inhibitor Panton-Valentine leukocyte inhibitor and -hemolysin AB causing the antibacterial activity of NETs to be ineffective in eliminating biofilm and even exacerbating biofilm infection (Bhattacharya et al., 2018)

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