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For selective chemical labeling of 5hmC, the fragmented DNA was incubated at 37 C for 2 h in a glucosylation buffer (50 mM HEPES buffer, 25 mM MgCl 2 , pH 8.0) containing -GT and N 3 -UDP-Glc

A potential obstacle to adopting a KD is however the experience of adverse symptoms in the first few weeks, often referred to as keto-flu. Symptoms of keto-flu may include headache, lightheadedness, fatigue, lethargy, brain fog, decreased exercise capacity, mood changes, constipation, muscle cramps, diarrhea and halitosis

Recently, glutamine metabolism has been shown to be linked to white adipose tissue (WAT) inflammation in obesity 110

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Furthermore, Tan et al

Protects DNA and slows the aging process