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2.1 The plasma free fatty acid profile and human -cell lipotoxicity in vivo Together with elevated concentrations of circulatory FFA, the plasma FFA profile has been shown to be associated with the development of T2D ( In the EPIC-InterAct case-cohort study ( in vitro ( in vivo , supporting what has previously been shown in vitro ( Further investigations are required to understand the effect of changes in FFA composition on human -cell function