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Although there is no reason to believe that a neutral antagonist would directly affect MC3R signaling, the possibility remains that such an antagonist may lead to cross-sensitization or reduced internalization of the dimer, in effect amplifying MC3R signaling further through increased surface expression or sensitization

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Measurements of intracellular ROS After treatment with IL-22 (25 ng/ml) and/or SFN (20 nM), HaCaT cells were harvested and stained with 2 M dihydroethidium (DHE, Beyotime Institute of Biotechnology) for 30 min at 37 C in the dark

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