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once a day for 6 consecutive days before induction of ischemia and at the beginning of reperfusion) significantly reduces the serum alanine aminotransferase (ALT) level in rats with ischemia-reperfusion model [6]

Chronic tiredness is defined as tiredness for more than 6 months

Then pull back to your exact target of 40 units and verify no bubbles remain

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doi:10.1024/0300-9831/a000602 Wang J, You D, Wang H, et al