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Without proper protection, this can lead to tanning, uneven tone, and stubborn dark spots

Cells were lysed using RIPA buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, and 0.1% sodium dodecyl sulfate, 2 mM DTT, 2 mM sodium orthovanadate, 1 protease inhibitor cocktail [Roche])

Trying to come up with your own protocol is unsafe, no matter what those guys in the peptide forum say

Always use sunscreen to protect your skin from UV rays and prevent pigmentation from returning

However, we also made several important additional observations

A transient peptide can produce a pulse of activity instead of a sustained alteration