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For experiments involving caspase-3/7 staining, CellEvent Caspase-3/7 Green Detection Reagent (Thermo Scientific C10723) and CPP-CAND were added to live cells 24 h before imaging using an Eclipse Ti-U inverted microscope (NIKON)

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References Sies, H

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TRAF6 is a central adaptive protein involved in toll-like receptor and interleukin-1 receptor (IL-1R) signaling leading to nuclear factor-kappa B (NF-B) activation, which is vital for immune cell development, activation, and tolerance