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Because each sequence in the initial alignment has a length of 30 amino acids and each position can be mutated to one of 19 amino acids (20 if the position is gapped), this gives 570 single-step mutants in the first generation for each sequence in the training set, that is, 71,304 sequences, reducing to 69,639 sequences after removing duplicates

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doi: 10.1007/s00592-017-0994-x 76 LingvayISumithranPCohenRVle RouxCW

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