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The supernatant was collected in a 1.5 mL Eppendorf microtube, processed for the extraction of various types of compounds listed below, and used for LC-MS measurement

doi:10.1016/j.gene.2021.145407 Chew A, Buck EA, Peretz S, Sirugo G, Rinaldo P, Isaya G

Yes, its good practice to let both the peptide vial and the bacteriostatic water come to room temperature before reconstituting

Parisi, U.G

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[DOI] [PMC free article] [PubMed] [Google Scholar] 21.Gudbergsen H, Overgaard A, Henriksen M, Whrens EE, Bliddal H, Christensen R, et al