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The germicidal activity of ethylene oxide, for example, arises from its ability to serve as a reactive alkylating agent

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However, KL1 did not appear to obliterate the immune response, and KL1 treatment alone did not cause bacterial outgrowth in either cell-based or murine infection models

10.1016/j.rmed.2015.08.008 155 Perez-VilarJ.OlsenJ

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After three washes with 200 l prewarmed PBS, 100 l of 300 M prewarmed Hanks balanced salt solution (Gibco) was added, and luminescence was immediately measured at 37 C using a Synergy H1 reader