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Then, 15 g of protein was mixed with NativePAGE sample buffer (Invitrogen), Coomassie G-250 and protease inhibitor, loaded into the lanes of a 312% Bis-Tris NativePAGE gel (Invitrogen) and run according to the manufacturers instructions

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These data points create a comprehensive picture of treatment effectiveness that goes beyond the scale

using an integrated strategy that combined structure-based virtual screening, chemical synthesis, and bioassay (Figure 6) (113)

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