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Pelleted cells were resuspended in Lysis A buffer (40 mM HEPES pH 7.5, 2 mM magnesium acetate, 200 mM NaCl, 10 mM DTT, 1 mM benzamidine, 10 g/mL leupeptin, 50 g/mL AEBSF), and stored at 20 C

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Methylated DNA immunoprecipitation (MeDIP) and hydroxymethylated DNA immunoprecipitation (hMeDIP) Purified genomic DNA was sonicated and subjected to agarose gel electrophoresis and DNA fragments between 150200 bp were recovered

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doi: Darling KA, Eggleston MJF, Retallick-Brown H, Rucklidge JJ