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If it is suspected that the protein is expressed at a low level, verification of correct expression can be accomplished using Western blotting with an antibody specific for either the target protein or GST
Lipotropic injections are generally not recommended during pregnancy or breastfeeding, for people with a known allergy to any component of the formulation (including B12/cobalt), for those with certain kidney or liver conditions, or for those with certain medical conditions
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Grassmann et al., 2015
