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The macrophage activation was analyzed by flow cytometry

Cell metabolism measurement OCR and ECAR in real time were measured by the Seahorse Bioscience extracellular flux analyzer (XF24, Seahorse Bioscience)

J Ovarian Res 17:27 Murri M, Luque-Ramrez M, Insenser M, Ojeda-Ojeda M, Escobar-Morreale HF (2013) Circulating markers of oxidative stress in PCOS: a systematic review and meta-analysis

Choid et al., 2018)

The FRCs are mainly located in the T cell and B cell zones, with high expression of podoplanin (PDPN) but diminished or absent CD31 endothelial cell marker gene expression (122)

If IGF-1 exceeds 3 standard deviations above the age-adjusted mean, reduce dose or discontinue