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The total recording should last 6 min, capturing the animals behavior during the experiment, but focusing on the last 4 min of immobility time

Yes, getting vitamin injections from a qualified healthcare provider is safe

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Evans SO, Khairuddin PF, Jameson MB (2017) Optimising selenium for modulation of cancer treatments

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L-Nle was preferred in the N-terminal for optimal inhibition and zinc coordination to the oxygen of the benzamide function is estimated to allow the lipophilic P1 substituent to be easily accommodated in the S1 pocket