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Mild side effects like slight redness or tiredness may occur but fade quickly

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Yeast deletion of MCK1 in the W303 background was performed by standard one-step gene replacement with PCR-generated cassettes (Longtine et al., 1998) using primers listed in Supplementary Table 2

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Because of its rapid onset of action (30 sec) and brief duration of action (~5 minutes), it is not recommended for maintenance therapy in myasthenia gravis (Drachman, 2012)