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Samples were then loaded on 10% SDSPAGE gels, transferred onto nitrocellulose membrane and stained with Ponceau S (Sigma) to visualize total protein loading

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Space these supplements at least 2 hours apart for optimal results

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Experimental data indicate that GHK-Cu can influence collagen and glycosaminoglycan synthesis, modulate proteoglycan and chondroitin sulfate turnover, and recruit fibroblasts, immune cells, and endothelial cells to sites of tissue injury

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