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Drawbacks of the 1ml approach Accuracy is the main concern
The sirtuins are NAD + -dependent deacetylases that are distributed in the nucleus (e.g., SIRT1, SIRT6, and SIRT7), the cytoplasm (e.g., SIRT2) and mitochondria (e.g., SIRT3-5), respectively
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NAD+ (Nicotinamide Adenine Dinucleotide) is a vital coenzyme that plays a key role in energy production, DNA repair, and overall cellular function
Keremi et al., 2009
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