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In clinical trials, severe gastrointestinal adverse reactions were reported more frequently among patients receiving RYBELSUS (7 mg 0.6%, 14 mg 2%) than placebo (0.3%)

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1 GLP-1R is involved in regulating both insulin and glucagon secretion, and its knockout can impair pancreatic function, thereby affecting the balance of these hormones and glucose homeostasis

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GSH or Ni-NTA bead washing was performed four times with cold PBS or washing buffer (20 mM Tris-Cl (pH 8.0), 300 mM NaCl, 20 mM imidazole)