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The subjects were given B12, folic acid, and other supplements, which dropped their homocysteine levels and both reduced disability from migraines as well as the pain

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Gehrig SM, van der Poel C, Hoeflich A, et al

For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35