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The DHA content was determined after adding 50 mM dithiothreitol (DTT) to the supernatants after the second centrifugation, according to the method of 2.4.3 Determination of the content of nitrogen compounds and chlorides The concentration of ammonium ion (NH 4 + ), nitrate (III) (NO 2 - ), nitrate (V) (NO 3 - ) and chlorides (Cl - ) was determined after extraction of lettuce samples in 2% acetic acid (puriss

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