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Salvi V, Barone-Adesi F, DAmbrosio V, Albert U, Maina G

After 2 h incubation, cells were washed twice with cold PBS and were extracted with 80% ice-cold methanol at 2 10 6 cells/mL

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The test item formulation was administered once each day by oral route for 11 consecutive days between 9:00 and 11:00 h