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If left unmonitored or untreated, approximately one-third of these chronically infected individuals will develop end-stage liver disease, such as cirrhosis or HBV-related HCC (HBV-HCC) 1,2,3,4 , which is associated with more than 600,000 deaths annually 5,6

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Then, membranes were incubated with appropriate secondary antibodies, HRP-goat-anti-mouse (Abcam, ab6789) or HRP-goat-anti-rabbit (Abcam, ab6721), to bind the primary antibodies for 1 h at room temperature

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