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The drug is common in the U.K

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Beads were washed with 15 bed volumes of 50 mM NaH 2 PO 4 pH 7.4, 25 mM imidazole, 150 mM NaCl, 5% glycerol followed by 15 bed volumes of 50 mM NaH 2 PO 4 pH 7.4, 25 mM imidazole, 300 mM NaCl, 5% glycerol

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