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Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)

However, ghrelin did not increase endogenous dopamine tone in the striatum

Transplantation 79 , 16911695 (2005)

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Maintain a healthy, sustainable diet : Focusing on incorporating a variety of whole foods while consuming enough protein will assist in muscle retention and fat regulation