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The procedure is carefully controlled for temperature and timing, with incubations typically ranging from 1 to 2 h at room temperature to overnight settings at 4C, depending on the target analyte

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Thiamine transport is also significantly reduced by bacterial lipopolysaccharide and in sepsis (163, 164)

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In addition, the markers of insulin metabolism (fasting glucose and insulin) and lipid profiles (low-density lipoprotein (LDL), high-density lipoprotein (HDL), cholesterol and triglyceride) were measured at the baseline as well as 6 weeks later