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After blocking, the membrane was incubated overnight at 4 C with the specified primary antibodies, including TRPM2 antibody at a dilution of 1:1000 and GAPDH antibody at a dilution of 1:3000

All four pairs of BsNbs exhibited the corresponding BsNb peak in chromatogram, and the conjugation efficiency was greater than 50% (Table S5)

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