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Approximately, half of the pelleted cells were lysed by incubation with 120 l CHAPS (Merck Millipore, including 0.15 units/l RiboLock [LifeTechnologies, Thermo Fisher Scientific]) on wet ice for 30 min and three short vortexes, thereafter the lysate was stored at 80 C

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It is commonly used to prevent bacterial, yeast, and mold growth

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