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3a,b,eh, Figs

Any references to tissues, pathways, or models are provided only for research context and do not imply therapeutic or clinical use

For a step-by-step technique, see standard patient education materials

The multifaceted mechanism of action of 5FU involves direct incorporation of its metabolites into DNA (FdUTP) and RNA (FUTP), as well as inhibition of thymidylate synthase (TS) via FdUMP 4,5 , making it more difficult to identify rational combination partners that would enhance clinical responses without eliciting adverse systemic toxicity

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