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doi: 10.1186/s11658-021-00271-y 24 FanZWirthAKChenDWruckCJRauhMBuchfelderMet al

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Results GSH depletion induces cell death in RPE cells To evaluate the effect of GSH depletion on the RPE cells, we used three different approaches: depletion of Cys 2 from cell culture media, treatment with buthionine sulphoximine (BSO, 1000 M) to block de novo synthesis of GSH, or treatment with erastin (10 M), an inhibitor of system x c 1

This finding strongly supports the necessity of RAMP1 and RAMP3 for mediating cagrilintide-induced weight loss through AMY1R and AMY3R signaling [2]

(Czechia) using validated analytical methods and laboratory testing protocols