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Navarro SL, Chang J-L, Peterson S, Chen C, King IB, Schwarz Y, et al

Blood 107:508513 Reyland ME (2009) Protein kinase C isoforms: multi-functional regulators of cell life and death

Additional Wnt3a results in indefinite growth of mouse intestinal organoids devoid of a functionally intact Wnt-secreting niche, necessitating the addition of exogenous Wnt3a to the expansion medium and the exclusion of Wnt3a from the differentiation medium

These are made by substituting some natural alpha amino acids with beta amino acids, that have the amino group attached to the beta carbon two atoms away from the carboxylate group

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