The reactions were terminated, and the enzyme kinetics of the conjugation of 1-chloro-2,4-dinitrobenzene to GSH was determined as previously described ( Histological Examination Lung tissues were collected and fixed in 4% paraformaldehyde, then embedded in paraffin using standard procedures, following by serially sectioned as previously described ( In Vitro Pull-Down Binding Assays To determine whether GSTP binds directly to HMGB1, recombinant polyhistidine-tagged GSTP (WT), GSTP (S184A), GSTP (S184D), and HMGB1 proteins were expressed in Escherichia coli BL21 (DE3) and purified through Ni-NTA spin column (Qiagen)
10.1161/01.res.86.11.1129 288 StuehrD
The sensitivity and 1specificity of the combined test were calculated at 0.78, with sensitivity, specificity, accuracy, positive predictive value, and negative predictive value being 68.75, 80.77, 76.50, 69.23, and 80.33, respectively
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