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The method consisted of a linear gradient from 100% buffer A (50 mM Na 3 PO 4 with 2M NaCl, pH 7.00) to 100% buffer B (50 mM Na 3 PO 4 , pH 7.00/ACN/IPA, 80/10/10, v/v/v) in 50 min with the flow rate of 1.0 mL/min and column temperature of 30 C

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