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doi: 10.2337/diabetes.53.9.2242

Immunohistochemistry After differentiation, cells were washed with PBS (Life technologies) and then fixed with ice-cold methanol (Sigma) for 10 minutes at 20 C

Some prodrugs require specific enzymes or endogenous compounds for hydrolysis, such as the carboxylesterases including acetylcholinesterase, butyrylcholinesterase and arylesterase that are critically involved in the bioconversion of ester-based prodrugs into pharmacologically active forms (Liederer and Borchardt, 2006)

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